Factor XIIIa Monoclonal antibody

Factor XIIIa Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2D2B2

Cat no : 66325-1-Ig

Synonyms

Coagulation factor XIIIa, F13A, F13A1, Factor XIIIA, Transglutaminase A chain

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Validation Data Gallery

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  WB analysis using 66325-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 66325-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit large intestine using 66325-1-Ig

  rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66325-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 66325-1-Ig

  rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig colon tissue, human placenta tissue, human peripheral blood platelets, rat colon tissue, rabbit large intestine tissue, pig liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66325-1-Ig targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

• Tested Reactivity: human, pig, mouse, rat, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Factor XIIIa fusion protein Ag11105
• Full Name: coagulation factor XIII, A1 polypeptide
• Calculated Molecular Weight: 732 aa, 83 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC027963
• Gene Symbol: Factor XIIIa
• Gene ID (NCBI): 2162
• RRID: AB_2881706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.

Publications

Species	Application	Title
human	WB,IHC	Inflamm Regen; Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling; Authors - Hirofumi Watanabe