Factor X Monoclonal antibody, PBS Only

Factor X Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D3F11

Cat no : 66753-1-PBS

Synonyms

coagulation factor X, F10, Factor X, FX, FXA, Stuart factor, Stuart Prower factor

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Validation Data Gallery

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  WB analysis of human plasma using 66753-1-Ig (same clone as 66753-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66753-1-Ig (Factor X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Factor X antibody (66753-1-Ig, Clone: 3D3F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66753-1-PBS targets Factor X in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Factor X fusion protein Ag11664
• Full Name: coagulation factor X
• Calculated Molecular Weight: 488 aa, 55 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC046125
• Gene Symbol: F10
• Gene ID (NCBI): 2159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.