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Factor X Monoclonal antibody

Factor X Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D3F11

Cat no : 66753-1-Ig

Synonyms

coagulation factor X, F10, Factor X, FX, FXA, Stuart factor, Stuart Prower factor

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Factor X Monoclonal antibody (66753-1-Ig)

    WB analysis of human plasma using 66753-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66753-1-Ig (Factor X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Factor X Monoclonal antibody (66753-1-Ig)

    IHC staining of human liver using 66753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Factor X Monoclonal antibody (66753-1-Ig)

    IHC staining of human liver using 66753-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Factor X Monoclonal antibody (66753-1-Ig)

    IF Staining of human liver using 66753-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Factor X antibody (66753-1-Ig, Clone: 3D3F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66753-1-Ig targets Factor X in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Factor X fusion protein Ag11664
Full Name coagulation factor X
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC046125
Gene Symbol F10
Gene ID (NCBI) 2159
RRIDAB_2882099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Protocols

Product Specific Protocols
WB protocol for Factor X antibody 66753-1-IgDownload protocol
IHC protocol for Factor X antibody 66753-1-IgDownload protocol
IF protocol for Factor X antibody 66753-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols