FXYD6 Monoclonal antibody, PBS Only

FXYD6 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H1B7

Cat no : 68058-1-PBS

Synonyms

FXYD6, Phosphohippolin

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Validation Data Gallery

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  WB analysis using 68058-1-Ig (same clone as 68058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68058-1-Ig (FXYD6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

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  WB analysis using 68058-1-Ig (same clone as 68058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68058-1-Ig (FXYD6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 68058-1-Ig (same clone as 68058-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using FXYD6 antibody (68058-1-Ig, Clone: 2H1B7 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68058-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68058-1-PBS targets FXYD6 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit, Chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FXYD6 fusion protein Ag8538
• Full Name: FXYD domain containing ion transport regulator 6
• Calculated Molecular Weight: 95 aa, 11 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC018652
• Gene Symbol: FXYD6
• Gene ID (NCBI): 53826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.