FXYD6 Monoclonal antibody
FXYD6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit, Chicken
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2H1B7
Cat no : 68058-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-12 cells, rat cerebellum tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse cerebellum tissue |
Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68058-1-Ig targets FXYD6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.
Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit, Chicken |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | FXYD6 fusion protein Ag8538 |
Full Name | FXYD domain containing ion transport regulator 6 |
Calculated Molecular Weight | 95 aa, 11 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC018652 |
Gene Symbol | FXYD6 |
Gene ID (NCBI) | 53826 |
RRID | AB_2918799 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.
Protocols
Product Specific Protocols | |
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WB protocol for FXYD6 antibody 68058-1-Ig | Download protocol |
IHC protocol for FXYD6 antibody 68058-1-Ig | Download protocol |
IF protocol for FXYD6 antibody 68058-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |