FXR1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FXR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13194-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13194-1-AP (same clone as 13194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 13194-1-AP (same clone as 13194-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 13194-1-AP (same clone as 13194-1-PBS)

  IP result of anti-FXR1 (IP:13194-1-AP, 3ug; Detection:13194-1-AP 1:800) with mouse skeletal muscle tissue lysate 8000ug. This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 13194-1-AP (same clone as 13194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 13194-1-AP (same clone as 13194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13194-1-AP (same clone as 13194-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (13194-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13194-1-AP (same clone as 13194-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 13194-1-PBS in a different storage buffer formulation.

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Product Information

13194-1-PBS targets FXR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FXR1 fusion protein Ag3873
• Full Name: fragile X mental retardation, autosomal homolog 1
• Calculated Molecular Weight: 621 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC028983
• Gene Symbol: FXR1
• Gene ID (NCBI): 8087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.