FXR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FXR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (1)

Cat no : 13194-1-AP

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1

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Validation Data Gallery

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  WB analysis using 13194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13194-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13194-1-AP

  IP result of anti-FXR1 (IP:13194-1-AP, 3ug; Detection:13194-1-AP 1:800) with mouse skeletal muscle tissue lysate 8000ug.

• 

  IHC staining of human heart using 13194-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13194-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13194-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (13194-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13194-1-AP

  Immunofluorescent analysis of HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, NIH/3T3 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• RIP: See 2 publications below

Product Information

13194-1-AP targets FXR1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FXR1 fusion protein Ag3873
• Full Name: fragile X mental retardation, autosomal homolog 1
• Calculated Molecular Weight: 621 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC028983
• Gene Symbol: FXR1
• Gene ID (NCBI): 8087
• RRID: AB_2110702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers; Authors - Yang Sun ; KD Validated
human	IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
mouse	IF	Neuron; Fmr1 KO and fenobam treatment differentially impact distinct synapse populations of mouse neocortex.; Authors - Gordon X Wang
mouse	IF	Nat Commun; Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle; Authors - Lance T. Denes
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang
		Elife; Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses.; Authors - Gordon X Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chao (Verified Customer) (01-30-2020)

Western blot show strong band in the basal state.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart