CoraLite®594-conjugated FUCA1 Monoclonal antibody

FUCA1 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G5C5

Cat no : CL594-67251

Synonyms

FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-67251

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of human liver cancer using CL594-67251

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200. DAPI (blue).

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  IF Staining of HepG2 using CL594-67251

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 FUCA1 antibody (CL594-67251, Clone: 1G5C5 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67251 targets FUCA1 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FUCA1 fusion protein Ag9638
• Full Name: fucosidase, alpha-L- 1, tissue
• Calculated Molecular Weight: 461 aa, 53 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC017338
• Gene Symbol: FUCA1
• Gene ID (NCBI): 2517
• RRID: AB_2920103
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).