FTO Monoclonal antibody, PBS Only

FTO Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Pig

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1E8B1

Cat no : 68111-1-PBS

Synonyms

KIAA1752, 1E8B1

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Validation Data Gallery

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  WB analysis using 68111-1-Ig (same clone as 68111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68111-1-Ig (same clone as 68111-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 68111-1-Ig (same clone as 68111-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68111-1-Ig (same clone as 68111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68111-1-Ig (same clone as 68111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 68111-1-Ig (same clone as 68111-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (68111-1-Ig, Clone:1E8B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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Product Information

68111-1-PBS targets FTO in Indirect ELISA, IHC, WB applications and shows reactivity with Human, Mouse, Pig samples.

• Tested Reactivity: Human, Mouse, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FTO fusion protein Ag26095
• Full Name: fat mass and obesity associated
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_001080432
• Gene Symbol: FTO
• Gene ID (NCBI): 79068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.