Filter: AllWBIHCFC (Intra)

WB analysis using 68111-1-Ig (same clone as 68111-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 68111-1-Ig (same clone as 68111-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 68111-1-Ig (same clone as 68111-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 68111-1-Ig (same clone as 68111-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 68111-1-Ig (same clone as 68111-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
FC experiment of SH-SY5Y using 68111-1-Ig (same clone as 68111-1-PBS)
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (68111-1-Ig, Clone:1E8B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.
×
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (68111-1-Ig, Clone:1E8B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

FTO Monoclonal antibody, PBS Only

FTO Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68111-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Pig

Applications

Indirect ELISA, IHC, WB

Synonyms

KIAA1752, 1E8B1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68111-1-PBS targets FTO in Indirect ELISA, IHC, WB applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity	Human, Mouse, Pig
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	FTO fusion protein Ag26095 Predict reactive species
Full Name	fat mass and obesity associated
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	NM_001080432
Gene Symbol	FTO
Gene ID (NCBI)	79068
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9C0B1
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.