FOXO1 Recombinant antibody, PBS Only (Capture)

FOXO1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230183G9

Cat no : 82997-2-PBS

Synonyms

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP00024-3

  Sandwich ELISA standard curve of MP00024-3, Human FOXO1 Recombinant Matched Antibody Pair - PBS only. 82997-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34671. 82997-5-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

• 

  Sample test of MP00024-3

  Serum of sixteen individual healthy human donors was measured. The human FOXO1 concentration of detected samples was determined to be 359.1 pg/mL with a range of 18.5 - 4,538.3 pg/mL.

• 

  Sample test of MP00024-3

  MCF-7, HEK-293T and C2C12 cell lysates were measured. The human FOXO1 concentration of detected samples was determined to be 416.1 pg/mL (based on a 1.2 mg/mL extract load) in MCF-7 cell lysate, 637.3 pg/mL (based on a 1.4 mg/mL extract load) in HEK-293T cell lysate and 1,050.2 pg/mL (based on a 3.2 mg/mL extract load) in C2C12 cell lysate.

• 

  Cytometric bead array standard curve of MP00024-2

  Cytometric bead array standard curve of MP00024-2, FOXO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82997-2-PBS. Detection antibody: 82997-3-PBS. Standard: Ag13296. Range: 1.25-20 ng/mL.

---

Product Information

82997-2-PBS targets FOXO1 as part of a matched antibody pair:

MP00024-2: 82997-2-PBS capture and 82997-3-PBS detection (validated in Cytometric bead array)

MP00024-3: 82997-2-PBS capture and 82997-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXO1 fusion protein Ag13296
• Full Name: forkhead box O1
• Calculated Molecular Weight: 655 aa, 70 kDa
• GenBank Accession Number: BC021981
• Gene Symbol: FOXO1
• Gene ID (NCBI): 2308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.