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FOXO1 Recombinant antibody, PBS Only (Detector)

FOXO1 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230183E3

Cat no : 82997-5-PBS

Synonyms

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00024-3

    Sandwich ELISA standard curve of MP00024-3

    Sandwich ELISA standard curve of MP00024-3, Human FOXO1 Recombinant Matched Antibody Pair - PBS only. 82997-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34671. 82997-5-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

  • Sample test of MP00024-3

    Sample test of MP00024-3

    Serum of sixteen individual healthy human donors was measured. The human FOXO1 concentration of detected samples was determined to be 359.1 pg/mL with a range of 18.5 - 4,538.3 pg/mL.

  • Sample test of MP00024-3

    Sample test of MP00024-3

    MCF-7, HEK-293T and C2C12 cell lysates were measured. The human FOXO1 concentration of detected samples was determined to be 416.1 pg/mL (based on a 1.2 mg/mL extract load) in MCF-7 cell lysate, 637.3 pg/mL (based on a 1.4 mg/mL extract load) in HEK-293T cell lysate and 1,050.2 pg/mL (based on a 3.2 mg/mL extract load) in C2C12 cell lysate.

Product Information

82997-5-PBS targets FOXO1 as part of a matched antibody pair:

MP00024-3: 82997-2-PBS capture and 82997-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXO1 fusion protein Ag13296
Full Name forkhead box O1
Calculated Molecular Weight 655 aa, 70 kDa
GenBank Accession NumberBC021981
Gene Symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.