FOXC2 Recombinant antibody, PBS Only (Detector)

FOXC2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240403H7

Cat no : 83476-5-PBS

Synonyms

MFH 1, Mesenchyme fork head protein 1, Forkhead-related protein FKHL14, Forkhead box protein C2, FKHL14

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00470-4

  Sandwich ELISA standard curve of MP00470-4, Human FOXC2 Recombinant Matched Antibody Pair - PBS only. 83476-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33121. 83476-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00470-4

  The mean FOXC2 concentration was determined to be 2.8 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  IF Staining of A375 using 83476-5-RR (same clone as 83476-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using FOXC2 antibody (83476-5-RR, Clone: 240403H7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83476-5-RR (same clone as 83476-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-5-RR, Clone:240403H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83476-5-RR (same clone as 83476-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-5-RR, Clone:240403H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83476-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83476-5-RR against Human FOXC2 were performed. The affinity constant is 2.79 nM.

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Product Information

83476-5-PBS targets FOXC2 as part of a matched antibody pair:

MP00470-4: 83476-7-PBS capture and 83476-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXC2 fusion protein Ag33121
• Full Name: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Calculated Molecular Weight: 54kd
• GenBank Accession Number: NM_005251
• Gene Symbol: FOXC2
• Gene ID (NCBI): 2303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.