FOX2/RBM9 Polyclonal antibody, PBS Only

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  WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

  WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 12498-1-AP (same clone as 12498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Cat No. 12498-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh

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Product Information

12498-1-PBS targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOX2/RBM9 fusion protein Ag3168
• Full Name: RNA binding motif protein 9
• Calculated Molecular Weight: 390 aa, 41 kDa
• Observed Molecular Weight: 50 and 60 kDa
• GenBank Accession Number: BC013115
• Gene Symbol: RBM9
• Gene ID (NCBI): 23543
• RRID: AB_2877861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43251
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)