FOX2/RBM9 Polyclonal antibody

• Featured Product
• KD/KO Validated

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (3)

Cat no : 12498-1-AP

Synonyms

FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh

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Validation Data Gallery

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  WB analysis using 12498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12498-1-AP

  WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells.

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  WB analysis using 12498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12498-1-AP

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug.

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  IP experiment of K-562 using 12498-1-AP

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug.

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  IHC staining of human breast cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, HEK-293 cells, Jurkat cells, NCCIT cells, HeLa cells, Neuro-2a cells
• Positive IP detected in: HepG2 cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human placenta tissue, mouse brain tissue, human ovary tumor tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

12498-1-AP targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOX2/RBM9 fusion protein Ag3168
• Full Name: RNA binding motif protein 9
• Calculated Molecular Weight: 390 aa, 41 kDa
• Observed Molecular Weight: 50 and 60 kDa
• GenBank Accession Number: BC013115
• Gene Symbol: RBM9
• Gene ID (NCBI): 23543
• RRID: AB_2877861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

Publications

Species	Application	Title
human	IHC	Front Pharmacol; Integrated pan-cancer analysis reveals the immunological and prognostic potential of RBFOX2 in human tumors; Authors - Fengxian Huang
human	WB,IF,CoIP	Nat Cell Biol; RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation; Authors - Xiaoyang Dou
human	WB	iScience; Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis; Authors - Yingchao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (12-19-2023)

Excellent antibody without non-specific bands. Works perfectly as described.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: A7R5 Smooth Muscle Cells

FOX2/RBM9 Antibody Western Blot validation (1:4000 dilution) in A7R5 Smooth Muscle Cells (Cat no:12498-1-AP)

FH

Gaurav (Verified Customer) (10-04-2023)

Excellent antibody for endogenous proteins and works very fine. The quality of results is up to the expectations in co-immunoprecipitation and western blotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: A7R5 smooth muscle cells (Rat)

FOX2/RBM9 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in A7R5 smooth muscle cells (Rat) (Cat no:12498-1-AP)

FH

Gaurav (Verified Customer) (10-04-2023)

Excellent antibody for endogenous proteins and works very fine. The quality of results is up to the expectations in co-immunoprecipitation and western blotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: A7R5 smooth muscle cells (Rat)

FOX2/RBM9 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in A7R5 smooth muscle cells (Rat) (Cat no:12498-1-AP)