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FLAD1 Monoclonal antibody, PBS Only

FLAD1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9A8

Cat no : 68491-1-PBS

Synonyms

FAD pyrophosphorylase, FAD synthase, FAD1, FADS, FLAD1, FMN adenylyltransferase, PP591, RP11 307C12.7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis using 68491-1-Ig (same clone as 68491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of U2OS using 68491-1-Ig (same clone as 68491-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

Product Information

68491-1-PBS targets FLAD1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FLAD1 fusion protein Ag6845
Full Name FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
Calculated Molecular Weight 446 aa, 49 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC011378
Gene Symbol FLAD1
Gene ID (NCBI) 80308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).