FLAD1 Monoclonal antibody

FLAD1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F9A8

Cat no : 68491-1-Ig

Synonyms

FAD pyrophosphorylase, FAD synthase, FAD1, FADS, FLAD1, FMN adenylyltransferase, PP591, RP11 307C12.7

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Validation Data Gallery

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  WB analysis using 68491-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 68491-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68491-1-Ig targets FLAD1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FLAD1 fusion protein Ag6845
• Full Name: FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
• Calculated Molecular Weight: 446 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC011378
• Gene Symbol: FLAD1
• Gene ID (NCBI): 80308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).