FKBP2 Recombinant antibody

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  WB analysis using 84077-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84077-6-RR (FKBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84077-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84077-6-RR (FKBP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84077-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84077-6-RR (FKBP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84077-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84077-6-RR against Human FKBP2 were performed. The affinity constant is below 1 pM.

FKBP2 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84077-6-RR

Clone No.241166B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 241166B9

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, K-562 cells, HuH-7 cells, NIH/3T3 cells, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84077-6-RR targets FKBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FKBP2 fusion protein Ag2255
• Full Name: FK506 binding protein 2, 13kDa
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC003384
• Gene Symbol: FKBP2
• Gene ID (NCBI): 2286
• RRID: AB_3671646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P26885
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant (PMID:8373365).