FITC Recombinant antibody, PBS Only

• 

  WB analysis using 80003-1-RR (same clone as 80003-1-PBS)

  FITC-conjugated secondary antibodies were subjected to SDS PAGE followed by western blot with 80003-1-RR (FITC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80003-1-PBS in a different storage buffer formulation.

FITC Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 80003-1-PBS

Clone No.1B2

Host / Isotype

Rabbit / IgG

Reactivity

chemical compound, fitc conjugated

Applications

WB, Indirect ELISA

Synonyms

Fluorescein, 5-Isothiocyanat Fluorescein

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Product Information

80003-1-PBS targets FITC in WB, Indirect ELISA applications and shows reactivity with chemical compound, fitc conjugated samples.

• Tested Reactivity: chemical compound, fitc conjugated
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Compound
• Full Name: FITC
• RRID: AB_2882935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.