Tested Applications
| Positive WB detected in | FITC conjugated antbody |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80003-1-RR targets FITC in WB, IF, IP, ELISA applications and shows reactivity with fitc conjugated samples.
| Tested Reactivity | fitc conjugated |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | FITC |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2882935 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FITC antibody 80003-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FITC Recombinant monoclonal antibody (1B2), catalog number 80003-1-RR, has 5 total citations. These appear in Emerg Microbes Infect, Acta Neuropathol Commun, Biomed Pharmacother, Drug Dev Res, and Genetics. Research fields span ferroptosis and respiratory epithelial barrier disruption, amyloid-β–driven olfactory dysfunction, sorafenib-resistant hepatocellular carcinoma, sevoflurane-induced cognitive dysfunction via NF-κB signaling, and yeast lifespan extension through APCCdh1 function.
| Species | Application | Title |
|---|---|---|
Emerg Microbes Infect Streptococcus suis Stk1 sensitizes epithelial cells to ferroptosis and exacerbates disruption of the respiratory epithelial barrier. | ||
Acta Neuropathol Commun Localized amyloid-β oligomers in the lateral entorhinal cortex drives olfactory dysfunction through aberrant neuronal and network activity. | ||
Biomed Pharmacother Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma | ||
Drug Dev Res Carnosol alleviates sevoflurane-induced cognitive dysfunction by mediating NF-κB pathway in aged rats | ||
Genetics Discovery of a small peptide that increases yeast lifespan by enhancing the function of APCCdh1 in nondividing quiescent cells. |
Reviews
What customers say
One reviewer (Nishita Shukla) rated this antibody 5/5 stars for Western Blot at a 1:1000 dilution using lot 230011415 on Saccharomyces cerevisiae samples. They reported the antibody worked perfectly for probing a FITC-tagged peptide, producing intense and clean signal with minimal off-target binding. Overall, the feedback is highly positive, confirming strong performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nishita (Verified Customer) (01-19-2026) | Antibody worked perfectly for probing a FITC-tagged peptide on a western blot. The signal was intense and clean, minimal off target binding.
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