FITC Recombinant antibody

FITC Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

fitc conjugated and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B2

Cat no : 80003-1-RR

Synonyms

Fluorescein, 5-Isothiocyanat Fluorescein

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Validation Data Gallery

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  WB analysis using 80003-1-RR

  FITC-conjugated secondary antibodies were subjected to SDS PAGE followed by western blot with 80003-1-RR (FITC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: FITC conjugated antbody

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below

Product Information

80003-1-RR targets FITC in WB, IP, ELISA applications and shows reactivity with fitc conjugated samples.

• Tested Reactivity: fitc conjugated
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Compound
• Full Name: FITC
• RRID: AB_2882935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This rabbit recombinant antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Publications

Species	Application	Title
human	IP	Biomed Pharmacother; Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma; Authors - Jialiang Luo