FIS1 Monoclonal antibody

FIS1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (5)







Cat no : 66635-1-Ig


CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66635-1-Ig targets FIS1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, goat, fish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FIS1 fusion protein Ag1409
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)


Product Specific Protocols
WB protocol for FIS1 antibody 66635-1-IgDownload protocol
IHC protocol for FIS1 antibody 66635-1-IgDownload protocol
IF protocol for FIS1 antibody 66635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Chun (Verified Customer) (06-19-2022)

This antibody worked very well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells and HEK293 cells