FIS1 Monoclonal antibody, PBS Only

FIS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10E1

Cat no : 66635-1-PBS

Synonyms

TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135

---

Validation Data Gallery

• 

  WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 66635-1-Ig (same clone as 66635-1-PBS)

  IP result of anti-FIS1 (IP:66635-1-Ig, 4ug; Detection:66635-1-Ig 1:1000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66635-1-Ig (same clone as 66635-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (66635-1-Ig, Clone: 2F10E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

---

Product Information

66635-1-PBS targets FIS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FIS1 fusion protein Ag1409
• Full Name: fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC009428
• Gene Symbol: FIS1
• Gene ID (NCBI): 51024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)