FHIT Recombinant antibody

FHIT Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240564D9

Cat no : 83710-1-RR

Synonyms

AP3Aase, AP3A hydrolase, Adenylylsulfate-ammonia adenylyltransferase, Adenylylsulfatase, Adenosine 5'-monophosphoramidase FHIT

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Validation Data Gallery

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  WB analysis using 83710-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83710-1-RR (FHIT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83710-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83710-1-RR against Human FHIT were performed. The affinity constant is 5.01 nM.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83710-1-RR is FHIT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FHIT fusion protein Ag4181
• Full Name: fragile histidine triad gene
• Calculated Molecular Weight: 147 aa, 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC032336
• Gene Symbol: FHIT
• Gene ID (NCBI): 2272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.