FGL2 Monoclonal antibody, PBS Only

FGL2 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C9C5

Cat no : 67152-1-PBS

Synonyms

FGL2, FGL-2, fibrinogen like 2, Fibrinogen like protein 2, Fibroleukin, pT49, T49

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Validation Data Gallery

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  WB analysis of THP-1 using 67152-1-Ig (same clone as 67152-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67152-1-Ig (same clone as 67152-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 67152-1-Ig (same clone as 67152-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 67152-1-Ig (same clone as 67152-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67152-1-PBS targets FGL2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGL2 fusion protein Ag28619
• Full Name: fibrinogen-like 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC017813
• Gene Symbol: FGL2
• Gene ID (NCBI): 10875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.