FGL2 Monoclonal antibody

FGL2 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse and More (1)

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2C9C5

Cat no : 67152-1-Ig

Synonyms

FGL2, FGL-2, fibrinogen like 2, Fibrinogen like protein 2, Fibroleukin, pT49, T49

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Validation Data Gallery

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  WB analysis of THP-1 using 67152-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67152-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 67152-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse spleen using 67152-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: THP-1 cells, Jurkat cells
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67152-1-Ig targets FGL2 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGL2 fusion protein Ag28619
• Full Name: fibrinogen-like 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC017813
• Gene Symbol: FGL2
• Gene ID (NCBI): 10875
• RRID: AB_2882449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.

Publications

Species	Application	Title
human	WB	J Clin Invest; Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma; Authors - Mingen Lin
mouse	WB	Cell Rep; FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation; Authors - Tongguan Tian