FGFR2 Polyclonal antibody, PBS Only

FGFR2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 13042-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13042-1-AP (same clone as 13042-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FGFR2 antibody (13042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13042-1-AP (FGFR2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using FGFR2 antibody 13042-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human FGFR2 (13042-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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Product Information

13042-1-PBS targets FGFR2 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGFR2 fusion protein Ag3701
• Full Name: fibroblast growth factor receptor 2
• Calculated Molecular Weight: 709 aa, 79 kDa
• GenBank Accession Number: BC039243
• Gene Symbol: FGFR2
• Gene ID (NCBI): 2263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.