FGFR2 Polyclonal antibody

FGFR2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 13042-1-AP

Synonyms

EC:2.7.10.1, CFD1, CEK3, CD332, BFR 1



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13042-1-AP targets FGFR2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGFR2 fusion protein Ag3701
Full Name fibroblast growth factor receptor 2
Calculated Molecular Weight 709 aa, 79 kDa
Observed Molecular Weight
GenBank Accession NumberBC039243
Gene Symbol FGFR2
Gene ID (NCBI) 2263
RRIDAB_10642943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.

Protocols

Product Specific Protocols
IHC protocol for FGFR2 antibody 13042-1-APDownload protocol
IF protocol for FGFR2 antibody 13042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Am J Cancer Res

miR-381-3p suppresses the proliferation of oral squamous cell carcinoma cells by directly targeting FGFR2.

Authors - Xiao Yang
mouse

Bioengineered

The effects of Omarigliptin on promoting osteoblastic differentiation.

Authors - Fake Liao
mouse

Nephron

The Effects of M1/M2 Macrophages on the mRNA Expression Profile of Diabetic Glomerular Endothelial Cells.

Authors - Yining Li
human

Anim Biotechnol

MiR-125b regulates the differentiation of hair follicles in Fine-wool Sheep and Cashmere goats by targeting MXD4 and FGFR2.

Authors - Haie Qu
human

Life (Basel)

MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion

Authors - Zhaodong Li
humanIF

Tissue Eng Part A

The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury

Authors - Limin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kus (Verified Customer) (12-08-2022)

Works good WB but could see working for Immunofluorescence.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
FH

K (Verified Customer) (02-02-2022)

This Ab works great for immunofluorescence and IHC at 1:200 dilution but this Ab did not work well for western blotting.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: HePG2, Hela