FGFR1 Monoclonal antibody, PBS Only

FGFR1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

10F12E7

Cat no : 60325-1-PBS

Synonyms

bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr

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Validation Data Gallery

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  WB analysis of MCF-7 using 60325-1-Ig (same clone as 60325-1-PBS)

  MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  IF Staining of ACHN using 60325-1-Ig (same clone as 60325-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed ACHN cells using FGFR1 antibody (60325-1-Ig, Clone: 10F12E7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60325-1-PBS targets FGFR1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGFR1 fusion protein Ag3646
• Full Name: fibroblast growth factor receptor 1
• Calculated Molecular Weight: 820 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC018128
• Gene Symbol: FGFR1
• Gene ID (NCBI): 2260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.