FGFR1 Monoclonal antibody, PBS Only (Detector)

FGFR1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

10F12E7

Cat no : 60325-1-PBS

Synonyms

EC:2.7.10.1, CEK, CD331, bFGF-R-1, BFGFR

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Validation Data Gallery

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  WB analysis of MCF-7 using 60325-1-Ig (same clone as 60325-1-PBS)

  MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60325-1-Ig (same clone as 60325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  IF Staining of ACHN using 60325-1-Ig (same clone as 60325-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed ACHN cells using FGFR1 antibody (60325-1-Ig, Clone: 10F12E7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 60325-1-Ig (same clone as 60325-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug FGFR1 Monoclonal antibody (60325-1-Ig, Clone:10F12E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60325-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50453-3

  Cytometric bead array standard curve of MP50453-3, FGFR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68982-1-PBS. Detection antibody: 60325-1-PBS. Standard:Ag31801. Range: 1.563-100 ng/mL.

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Product Information

60325-1-PBS targets FGFR1 as part of a matched antibody pair:

MP50453-3: 68982-1-PBS capture and 60325-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGFR1 fusion protein Ag3646
• Full Name: fibroblast growth factor receptor 1
• Calculated Molecular Weight: 820 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC018128
• Gene Symbol: FGFR1
• Gene ID (NCBI): 2260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.