FGF18 Monoclonal antibody

FGF18 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D11D12

Cat no : 60341-1-Ig

Synonyms

FGF 18, FGF18, fibroblast growth factor 18, ZFGF5

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Validation Data Gallery

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  WB analysis of HT-1080 using 60341-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 60341-1-Ig (FGF18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60341-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60341-1-Ig (FGF18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HT-1080 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60341-1-Ig targets FGF18 in WB, ELISA applications and shows reactivity with human, mouse, rabbit samples.

• Tested Reactivity: human, mouse, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGF18 fusion protein Ag2041
• Full Name: fibroblast growth factor 18
• Calculated Molecular Weight: 207 aa, 24 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC006245
• Gene Symbol: FGF18
• Gene ID (NCBI): 8817
• RRID: AB_2881450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.; Authors - Kai Guo