FEM1C antibody (14037-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 14037-1-AP (same clone as 14037-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 14037-1-AP (same clone as 14037-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

14037-1-PBS targets FEM1C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FEM1C fusion protein Ag5099 Predict reactive species
Full Name	fem-1 homolog c (C. elegans)
Calculated Molecular Weight	69 kDa
Observed Molecular Weight	69 kDa
GenBank Accession Number	BC028369
Gene Symbol	FEM1C
Gene ID (NCBI)	56929
RRID	AB_2878002
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96JP0
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.