FEM1C Polyclonal antibody, PBS Only

FEM1C Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14037-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 14037-1-AP (same clone as 14037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14037-1-AP (same clone as 14037-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

---

Product Information

14037-1-PBS targets FEM1C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FEM1C fusion protein Ag5099
• Full Name: fem-1 homolog c (C. elegans)
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC028369
• Gene Symbol: FEM1C
• Gene ID (NCBI): 56929
• RRID: AB_2878002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.