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FEM1C Polyclonal antibody

FEM1C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14037-1-AP

Synonyms

Protein fem-1 homolog C, FEM1-gamma, FEM1A, FEM1 gamma, FEM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis using 14037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of HepG2 using 14037-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of HepG2 using 14037-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse brain using 14037-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse brain using 14037-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse heart using 14037-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse heart using 14037-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of human heart using 14037-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of mouse brain using 14037-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of mouse brain using 14037-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of human heart using 14037-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FEM1C Polyclonal antibody (14037-1-AP)

    IF Staining of HeLa using 14037-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, HepG2 cells, rat brain tissue
Positive IHC detected inhuman heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14037-1-AP targets FEM1C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEM1C fusion protein Ag5099
Full Name fem-1 homolog c (C. elegans)
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC028369
Gene Symbol FEM1C
Gene ID (NCBI) 56929
RRIDAB_2878002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.

Protocols

Product Specific Protocols
WB protocol for FEM1C antibody 14037-1-APDownload protocol
IHC protocol for FEM1C antibody 14037-1-APDownload protocol
IF protocol for FEM1C antibody 14037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols