CoraLite® Plus 488-conjugated FDPS Monoclonal antibody

FDPS Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B8A7

Cat no : CL488-67972

Synonyms

Farnesyl diphosphate synthase, EC:2.5.1.10, EC:2.5.1.1, Dimethylallyltranstransferase, 3B8A7

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Validation Data Gallery

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  WB analysis using CL488-67972

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67972 (FDPS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL488-67972

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FDPS (CL488-67972, Clone:3B8A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67972 targets FDPS in WB, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FDPS fusion protein Ag9247
• Full Name: farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
• Calculated Molecular Weight: 419 aa, 48 kDa
• Observed Molecular Weight: 38-39 kDa
• GenBank Accession Number: BC010004
• Gene Symbol: FDPS
• Gene ID (NCBI): 2224
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C