FBXO6 Monoclonal antibody, PBS Only

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• KD/KO Validated

FBXO6 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B7F9

Cat no : 67697-1-PBS

Synonyms

F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6

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Validation Data Gallery

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  WB analysis using 67697-1-Ig (same clone as 67697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67697-1-Ig (FBXO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67697-1-Ig (same clone as 67697-1-PBS)

  WB result of FBXO6 antibody (67697-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO6 transfected Jurkat cells. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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Product Information

67697-1-PBS targets FBXO6 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FBXO6 fusion protein Ag30512
• Full Name: F-box protein 6
• Calculated Molecular Weight: 293 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020880
• Gene Symbol: FBXO6
• Gene ID (NCBI): 26270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).