FBXO6 Monoclonal antibody

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  WB analysis using 67697-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67697-1-Ig (FBXO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67697-1-Ig

  WB result of FBXO6 antibody (67697-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO6 transfected Jurkat cells.

• Featured Product
• KD/KO Validated

FBXO6 Monoclonal Antibody for WB, ELISA

Cat No. 67697-1-Ig

Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

CoraLite® Plus 488

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, MOLT-4 cells, K-562 cells, U-937 cells, RAW 264.7 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67697-1-Ig targets FBXO6 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FBXO6 fusion protein Ag30512
• Full Name: F-box protein 6
• Calculated Molecular Weight: 293 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020880
• Gene Symbol: FBXO6
• Gene ID (NCBI): 26270
• RRID: AB_2882889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRD1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).

Publications

Species	Application	Title
human	WB	Front Oncol; Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer; Authors - Pengyu Fan
human	WB	J Control Release; Delivery of FBXO6 with highly branched poly(β-amino ester)s to modulate the inflammatory environment for the treatment of osteoarthritis; Authors - Yongxiang Shi