FBXO44 Recombinant antibody

FBXO44 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230229G7

Cat no : 82857-2-RR

Synonyms

FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44

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Validation Data Gallery

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  WB analysis using 82857-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82857-2-RR (FBXO44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 82857-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82857-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 82857-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 82857-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of A431 using 82857-2-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug FBXO44 Recombinant antibody (82857-2-RR, Clone:230229G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82857-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82857-2-RR against Human FBXO44 were performed. The affinity constant is 0.134 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells, A549 cells, HeLa cells, HEK-293 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, U2OS cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82857-2-RR targets FBXO44 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FBXO44 fusion protein Ag0977
• Full Name: F-box protein 44
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC007832
• Gene Symbol: FBXO44
• Gene ID (NCBI): 93611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)