FBXO32 Recombinant antibody

FBXO32 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230243H7

Cat no : 82941-1-RR

Synonyms

F-box only protein 32, F box protein 32, F box only protein 32, Atrogin-1, Atrogin 1

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Validation Data Gallery

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  IHC staining of mouse skeletal muscle using 82941-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82941-1-RR (FBXO32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 82941-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using FBXO32 antibody (82941-1-RR, Clone: 230243H7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-251 using 82941-1-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human FBXO32 (82941-1-RR, Clone:230243H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 82941-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82941-1-RR against Human FBXO32 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82941-1-RR targets FBXO32 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FBXO32 fusion protein Ag29394
• Full Name: F-box protein 32
• Calculated Molecular Weight: 355 aa, 42 kDa
• GenBank Accession Number: BC024030
• Gene Symbol: FBXO32
• Gene ID (NCBI): 114907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.