FBXO32 Recombinant antibody, PBS Only

FBXO32 Recombinant Antibody for IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230243H7

Cat no : 82941-1-PBS

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein

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Validation Data Gallery

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  IF Staining of C2C12 using 82941-1-RR (same clone as 82941-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using FBXO32 antibody (82941-1-RR, Clone: 230243H7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 82941-1-RR (same clone as 82941-1-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human FBXO32 (82941-1-RR, Clone:230243H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82941-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82941-1-PBS targets FBXO32 in IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FBXO32 fusion protein Ag29394
• Full Name: F-box protein 32
• Calculated Molecular Weight: 355 aa, 42 kDa
• GenBank Accession Number: BC024030
• Gene Symbol: FBXO32
• Gene ID (NCBI): 114907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.