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FBXO17 Monoclonal antibody, PBS Only

FBXO17 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3D3

Cat no : 68246-1-PBS

Synonyms

F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300

Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis of NCI-H1299 using 68246-1-Ig (same clone as 68246-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis of A549 using 68246-1-Ig (same clone as 68246-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis using 68246-1-Ig (same clone as 68246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    IF Staining of HepG2 using 68246-1-Ig (same clone as 68246-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (68246-1-Ig, Clone: 1F3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68246-1-PBS targets FBXO17 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBXO17 fusion protein Ag3867
Full Name F-box protein 17
Calculated Molecular Weight 278 aa, 31 kDa
Observed Molecular Weight29-31 kDa
GenBank Accession NumberBC012385
Gene Symbol FBXO17
Gene ID (NCBI) 115290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.