FBXO17 Monoclonal antibody

FBXO17 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3D3

Cat no : 68246-1-Ig

Synonyms

F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300

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Validation Data Gallery

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  WB analysis of NCI-H1299 using 68246-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68246-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68246-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68246-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (68246-1-Ig, Clone: 1F3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, U2OS cells, A549 cells, SH-SY5Y cells, U-87 MG cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68246-1-Ig targets FBXO17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FBXO17 fusion protein Ag3867
• Full Name: F-box protein 17
• Calculated Molecular Weight: 278 aa, 31 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC012385
• Gene Symbol: FBXO17
• Gene ID (NCBI): 115290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.