FAT1 Polyclonal antibody, PBS Only

FAT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30733-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30733-1-AP (same clone as 30733-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30733-1-AP (FAT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30733-1-AP (same clone as 30733-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30733-1-AP (FAT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 30733-1-AP (same clone as 30733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 30733-1-AP (FAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 30733-1-AP (same clone as 30733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 30733-1-AP (FAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30733-1-PBS in a different storage buffer formulation.

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Product Information

30733-1-PBS targets FAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAT1 fusion protein Ag33957
• Full Name: FAT tumor suppressor homolog 1 (Drosophila)
• Observed Molecular Weight: 506-600 kDa
• Gene Symbol: FAT1
• Gene ID (NCBI): 2195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379).