CoraLite®594-conjugated FARSB Monoclonal antibody

• Featured Product
• KD/KO Validated

FARSB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H10C2

Cat no : CL594-67924

Synonyms

Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB

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Validation Data Gallery

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  IF Staining of U-251 using CL594-67924

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using CoraLite®594 FARSB antibody (CL594-67924, Clone: 1H10C2 ) at dilution of 1:200.

• 

  IF Staining of U-251 using CL594-67924

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using CoraLite®594 FARSB antibody (CL594-67924, Clone: 1H10C2 ) at dilution of 1:200.

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  FC experiment of U-251 using CL594-67924

  1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated FARSB Monoclonal antibody (CL594-67924, Clone:1H10C2)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67924 targets FARSB in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FARSB fusion protein Ag9695
• Full Name: phenylalanyl-tRNA synthetase, beta subunit
• Calculated Molecular Weight: 589 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC017783
• Gene Symbol: FARSB
• Gene ID (NCBI): 10056
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.