FAP Recombinant antibody

FAP Uni-rAb^TM Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

241129B4

Cat no : 84018-4-RR

Synonyms

FAPA, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form

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Validation Data Gallery

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  WB analysis of U-87 MG using 84018-4-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Transfected HEK-293T using 84018-4-RR

  1x10^6 HEK-293T cells or Transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 84018-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

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Tested Applications

• Positive WB detected in: U-87 MG cells
• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84018-4-RR is FAP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: fibroblast activation protein, alpha
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_004460.4
• Gene Symbol: FAP
• Gene ID (NCBI): 2191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).