FANCD2 Recombinant antibody, PBS Only

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  WB analysis using 83946-4-RR (same clone as 83946-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCD2 antibody (83946-4-RR, Clone: 241048G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83946-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

FANCD2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83946-4-PBS

Clone No.241048G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241048G8, FA4, FACD, FAD, FAD2

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Product Information

83946-4-PBS targets FANCD2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FANCD2 fusion protein Ag29460
• Full Name: Fanconi anemia, complementation group D2
• Calculated Molecular Weight: 164 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: NM_001018115
• Gene Symbol: FANCD2
• Gene ID (NCBI): 2177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9BXW9
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)