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FANCD2 Recombinant antibody

FANCD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241048G8

Cat no : 83946-4-RR

Synonyms

FANCD, FAD2, FAD, FACD, FA4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FANCD2 Recombinant antibody (83946-4-RR)

    WB analysis using 83946-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using FANCD2 Recombinant antibody (83946-4-RR)

    FC experiment of HeLa using 83946-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83946-4-RR

    Affinity and Kinetic Characterization of 83946-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, Jurkat cells, U2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83946-4-RR is FANCD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FANCD2 fusion protein Ag29460
Full Name Fanconi anemia, complementation group D2
Calculated Molecular Weight 164 kDa
Observed Molecular Weight150-160 kDa
GenBank Accession NumberNM_001018115
Gene Symbol FANCD2
Gene ID (NCBI) 2177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)

Protocols

Product Specific Protocols
WB protocol for FANCD2 antibody 83946-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols