FAM114A1 Monoclonal antibody, PBS Only (Capture)

FAM114A1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11D6

Cat no : 67926-1-PBS

Synonyms

Protein NOXP20, Protein FAM114A1, NOXP20, Nervous system overexpressed protein 20, 1B11D6

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Validation Data Gallery

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  WB analysis using 67926-1-Ig (same clone as 67926-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67926-1-Ig (same clone as 67926-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67926-1-Ig (same clone as 67926-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 67926-1-Ig (same clone as 67926-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67926-1-Ig (same clone as 67926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67926-1-Ig (FAM114A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67926-1-Ig (same clone as 67926-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (67926-1-Ig, Clone: 1B11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51192-1

  Cytometric bead array standard curve of MP51192-1, FAM114A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67926-1-PBS. Detection antibody: 67926-2-PBS. Standard:Ag17289. Range: 0.195-50 ng/mL.

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Product Information

67926-1-PBS targets FAM114A1 as part of a matched antibody pair:

MP51192-1: 67926-1-PBS capture and 67926-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAM114A1 fusion protein Ag17289
• Full Name: family with sequence similarity 114, member A1
• Calculated Molecular Weight: 563 aa, 61 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC040452
• Gene Symbol: FAM114A1
• Gene ID (NCBI): 92689
• RRID: AB_2918678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.