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FAM114A1 Monoclonal antibody

FAM114A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B11D6

Cat no : 67926-1-Ig

Synonyms

FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis using 67926-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis of NIH/3T3 using 67926-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis of U2OS using 67926-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis of PC-12 using 67926-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM114A1 Monoclonal antibody (67926-1-Ig)

    IHC staining of mouse brain using 67926-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67926-1-Ig (FAM114A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    IF Staining of HepG2 using 67926-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (67926-1-Ig, Clone: 1B11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, PC-12 cells, NIH/3T3 cells, A549 cells, HepG2 cells, LNCaP cells, HeLa cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67926-1-Ig targets FAM114A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FAM114A1 fusion protein Ag17289
Full Name family with sequence similarity 114, member A1
Calculated Molecular Weight 563 aa, 61 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC040452
Gene Symbol FAM114A1
Gene ID (NCBI) 92689
RRIDAB_2918678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.

Protocols

Product Specific Protocols
WB protocol for FAM114A1 antibody 67926-1-IgDownload protocol
IHC protocol for FAM114A1 antibody 67926-1-IgDownload protocol
IF protocol for FAM114A1 antibody 67926-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

RAB42 Promotes Glioma Pathogenesis via the VEGF Signaling Pathway.

Authors - Baoling Liu
View Article