FAH Monoclonal antibody

FAH Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8E11

Cat no : 67986-1-Ig

Synonyms

Fumarylacetoacetate hydrolase, FAA, EC:3.7.1.2, Beta-diketonase, 2F8E11

---

Validation Data Gallery

• 

  WB analysis using 67986-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of pig liver using 67986-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67986-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 67986-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67986-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 67986-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

---

Tested Applications

• Positive WB detected in: LNCaP cells, pig liver tissue, rat liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67986-1-Ig targets FAH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAH fusion protein Ag6704
• Full Name: fumarylacetoacetate hydrolase (fumarylacetoacetase)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC002527
• Gene Symbol: FAH
• Gene ID (NCBI): 2184
• RRID: AB_2918735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).