CoraLite® Plus 488-conjugated FAF2 Monoclonal antibody

• Featured Product
• KD/KO Validated

FAF2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H6D2

Cat no : CL488-66629

Synonyms

ETEA, 3H6D2, Protein ETEA, KIAA0887, FAS-associated factor 2

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Validation Data Gallery

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  WB analysis using CL488-66629

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66629 (FAF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66629 targets FAF2 in WB applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAF2 fusion protein Ag21536
• Full Name: Fas associated factor family member 2
• Calculated Molecular Weight: 445 aa, 53 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC014001
• Gene Symbol: FAF2
• Gene ID (NCBI): 23197
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.