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FADS2-Specific Recombinant antibody

FADS2-Specific Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241863G3

Cat no : 84527-5-RR

Synonyms

FADS2, EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FADS2-Specific Recombinant antibody (84527-5-RR)

    IF Staining of HepG2 using 84527-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FADS2-Specific antibody (84527-5-RR, Clone: 241863G3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84527-5-RR is FADS2-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FADS2-Specific fusion protein Ag27715
Full Name fatty acid desaturase 2
Calculated Molecular Weight 445 aa, 49 kDa
GenBank Accession NumberBC009011
Gene Symbol FADS2
Gene ID (NCBI) 9415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.

Protocols

Product Specific Protocols
IF protocol for FADS2-Specific antibody 84527-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols