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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FADS2-Specific Recombinant antibody (84527-5-RR)

    IF Staining of HepG2 using 84527-5-RR (same clone as 84527-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FADS2-Specific antibody (84527-5-RR, Clone: 241863G3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84527-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84527-5-RR

    Affinity and Kinetic Characterization of 84527-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84527-5-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

FADS2-Specific Recombinant antibody, PBS Only

FADS2-Specific {ptg:Brand} Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84527-5-PBS
Clone No.241863G3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

FADS2, EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

84527-5-PBS targets FADS2-Specific in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FADS2-Specific fusion protein Ag27715 Predict reactive species
Full Name fatty acid desaturase 2
Calculated Molecular Weight 445 aa, 49 kDa
GenBank Accession NumberBC009011
Gene Symbol FADS2
Gene ID (NCBI) 9415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95864
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.