FADD Recombinant antibody, PBS Only

FADD Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241976A11

Cat no : 84619-5-PBS

Synonyms

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein, 241976A11

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Validation Data Gallery

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  WB analysis using 84619-5-RR (same clone as 84619-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84619-5-RR (FADD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84619-5-RR (same clone as 84619-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (84619-5-RR, Clone: 241976A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 84619-5-RR (same clone as 84619-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

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  FC experiment of HT-1080 using 84619-5-RR (same clone as 84619-5-PBS)

  1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84619-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84619-5-RR against Human FADD were performed. The affinity constant is 19.8 nM.

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Product Information

84619-5-PBS targets FADD in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FADD fusion protein Ag6701
• Full Name: Fas (TNFRSF6)-associated via death domain
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC000334
• Gene Symbol: FADD
• Gene ID (NCBI): 8772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)